FBA | Fructose-bisphosphate aldolase class 2

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AS13 2731  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Synechocystis PCC6803


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Item No:
AS13 2731

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product information
Background FBP aldolase (FBP) is an enzyme (EC= which is catalyzing the aldol condensation of of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis. It belongs to class II fructose-bisphosphate aldolase family. Alternative names: FBPA, fructose-1,6-bisphosphate aldolase, fructose-bisphosphate aldolase class II.

Recombinant FBA from Synechocystis sp. PCC6803, UniProt: Q55664, Cyanobase: sll0018

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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AS16 3848 | Anti-FBA | Fructose-bisphosphate aldolase 1, cytoplasmic, rabbit antibodies

collection of antibodies to proteins involved in carbohydrate metabolism

Algal protein extraction buffer

Secondary antibodies

Additional information This antibody can be used as a marker of cytoplasmic fraction in cyanobacteria.
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

38.9 kDa

Confirmed reactivity Synechocystis PCC6803
Predicted reactivity Cyanobacteria
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in November 2013.

application example
western blot using anti-FBA antibodies

From 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 (A) extracted with SDS-sample buffer and respective amounts of recombinant FBA (B) were separated on 15 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T  for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL according to the manufacturer's instructions. Exposure time was  seconds.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA


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