SPS | Sucrose phosphate synthase (maize)
AS06 185 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Z.mays, S.lycopersicum | cellular [compartment marker] of cytoplasm
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Synthetic peptide derived from Zea mays SPS protein sequence (P31927).
120 | ~130 for Zea mays
10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).
Total IgG concentration is 3 µg/µl
SPS (sucrose phosphate synthase, EC 18.104.22.168) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J Plant Growth Regul. DOI 10.1007/s00344-014-9441-1. (immunolocalization)