ACT | Actin

AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. stolonifera cv. ‘Penncross’,  A. thaliana, Glycine max, H. vulgare, S. lycopersicum, S. tuberosum, Z. mays

ACT | Actin in the group Plant/Algal Antibodies / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS13 2640)


272 €
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product information

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.


ca. 100 amino acids of recombinant actin conserved more than 80 %  in Arabidopsis thaliana: actin-1 P0CJ46  AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293  AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications immunofluorescence (IF), Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire.
application information
Recommended dilution 1 : 250 (IF), 1 : 3000-1 : 5000 (WB)
Expected | apparent MW

41.6 | 45 kDa

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Cynara cardunculus, Glycine max, Hordeum vulgare, Solanum tuberosum, Zea mays
Predicted reactivity

Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cucumis sativus, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum,  Vicia faba

Not reactive in

Chlamydomonas reinhardtii (too high background for this species)

Additional information

This product can be sold containing ProClin if requested

Selected references Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Qiu et al. (2015). Soy 14-3-3 protein SGF14c, a new regulator of tolerance to salt–alkali stress. Plant Biotechnology Reports pp 1-9.
Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.
Buxa et al. (2015). Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. ront Plant Sci. 2015; 6: 650. Published online 2015 Aug 19.
Zheng et al. (2014). iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res. 2014 May 13.

Application example

western blot using anti-plant actin antibody

15 µg of total protein extracted with PEB (AS08 300) from  leaf tissue of (1) Arabidopsis thaliana, (2) Hordeum vulgare, (3) Zea mays were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-actin (AS13 2640, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, recommended secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with ECL Advance (GE Healthcare) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.


 immunolocalization using anti-plant actin antibody

Actin cytoskeleton in 5 days old Arabidopsis thaliana seedlings. Actin signal shown in green, PIN1 in red and DAPI in blue. The material has been fixed in 2 % formaldehyde for 45 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-actin primary antibody was diluted in 1:250 and anti-rabbit Alexa 488 and Alexa 555 were both diluted in 1: 500 (Invitrogen). Scale bar - 20 µm.

Courtesy: Dr. Taras Pasternak, Freiburg University, Germany

Western blot of anti-actin antibodies on potato tuber flesh

Proteins were extracted from tuber flesh of Russet Burbank potato (Solanum tuberosum) with 0.1 M Tris HCl (pH=8.0), 5% sucrose (m/v), 2% (m/v) SDS, protease inhibitors (PMSF 1mM). Samples were heated 95°C 5 min, and 10 μg of total protein was resolved in 12% SDS PAGE and blotted to PVDF membrane for 1h-1.5h using tank transfer. Blots were blocked with a skimmed milk 4% (m/v) in T-TBS (1.5h) at RT with agitation. Primary antibodies (AS13 2640) were applied overnight +4°C in dilution 1:5000 with agitation. After washing with T-TBS 2-3 times, membrane was incubated with secondary antibodies (Goat Anti-Rabbit HRP conjugate, Transgen biotech HS101) 1:10000 for 1 hour at RT. Blot was washed as above and developed with ECL (Clarity Western ECL Substrate, BioRad, 170-5060) for 5 – 10 minutes. Exposure time – 20.395 seconds.

Courtesy of Iauhenia Isayenka, University of Sherbrooke, Canada

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