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AGO set
Tag Antibody Set 

FtsZ | Procaryotic cell division GTPase (cyanobacterial)

AS07 217  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Cyanobacteria

FtsZ | Procaryotic cell division GTPase (cyanobacterial) in the group Antibodies for Plant/Algal  / Developmental Biology / Cytoskeleton at Agrisera AB (Antibodies for research) (AS07 217)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Customer reviews

Product Information

Immunogen

Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein Q3MC27 overexpressed in E.coli.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

44.5 | 50 kDa

Reactivity

Confirmed reactivity Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Predicted reactivity

Phaeodactylum tricornutum, Prochlorococcus sp. 

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

Western blot using anti-cyanobacterial FtsZ antibodies

Total protein samples (5 or 10 µg) from: Arabidopsis thaliana, leaf (1), Synechocystis 6803 motile (2)Synechocystis 6803 GT (glucose tolerant strain) (3), Synechococcus elongates 7942 (4), Marker - Pierce™ Prestained Protein MW Marker (kat #26612) were extracted with buffer (10mM Tris HCl, pH 8.0, 0.5% LDS, 4% glycerol, 0.1 mM EDTA ) were mixed with sample buffer and denatured for 5 min at 95°C. Samples were separated on 10% SDS -PAGE and blotted 1h to nitrocellulose membrane (Amersham Protran) using sem i-dry transfer (Bio -Rad) in standard transfer buffer in presence of 10% methanol. Transfer of proteins to the membrane was checked using 0,5% Ponceau S staining before the blocking step. Blots were blocked in buffer (2% low -fat milk in 1xPBS, 0,1% Tween) for 1h at room temperature (RT) with agitation. Blots were incubated in the primary antibody at a dilution of 1: 1000 at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS -T at RT with agitation. Blot was incubated in secondary antibody ( goat anti -rabbit IgG, AS09 602, Agrisera) diluted to 1:30 000 for 1h at RT with agitation. The blot was washed as above and developed for 5 min with C larity Western ECL Substrate and ChemiDoc detection system.

Courtesy Dr. Elena Pojidaeva, Laboratory of Plant Gene Expression, Timiryazev Institute of Plant Physiology RAS, 127276 Moscow Russia

Additional information

Additional information
To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody

To detect FtsZ protein in higher plants following antibodies are recommended:
AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodies
AS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies


This antibody can be used as a loading control antibody in cyanobacteria. 
Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30°C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).

Related products

Related products
AS09 413 | Anti-FtsZ1 and 2 | plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody
AS10 715 | Anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibody
AS13 2651 | Anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibody

Background

Background

FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.

Product citations

Selected references MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571
Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.
Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)
Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.