Tubulin beta chain
AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa
|Recommended dilution||1 : 1000 (IF), 1 : 500 (WB)|
|Expected | apparent MW||
49 | 49 kDa (Arabidopsis thaliana)
|Confirmed reactivity||Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa|
|Predicted reactivity||Brassica napus, Glycine max, Hordeum vulgare, Micromonoas pusilla, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrella patens, Populus trichocarpa, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vitis vinifera|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113|
Tubulin beta localization in Arabidopsis thaliana epidermis cells. Tubulin beta localized to division plates visualized in green, nucleus with DAPI in blue. Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin beta priamry antibody in dilution 1: 1000 and Agrisera goat anti-rabbit IgG DyLight® 488, secondary antibody in dilution 1: 2000. Scale bar – 10 µm.
(A) The 3 days old suspension cells of Arabidopsis thaliana have been fixed for 25 minutes with 2% FA, cell wall was partaillly digested with 0.2% Dricelase in MES buffer (pH 5.1) for 30 min at 37°C and the cells were dried on coated slide to omit PM extraction. Following by incubation for 45 minutes with Agrisera anti-tubulin beta chain primary antibody in a dilution of 1: 1000, washing once and 45 min incubation with a secondary goat anti-rabbit Alexa 555 antibody, in a dilution of 1:1000 dilution (Invitrogen). Cells were counterstained witn DAPI and visialized under epi/fluiresecnet microscope (B).
Courtesy Dr. Taras Pasternak, Freiburg University, Germany
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