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AGO set
Tag Antibody Set 

SUMO1 | Small ubiquitin-like modifier protein 1

AS08 308 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

SUMO1 | Small ubiquitin-like modifier protein 1 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS08 308)

PRODUCT INFORMATION IN PDF

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381 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag

Host Rabbit
Clonality Polyclonal
Purity Total purifed IgG
Format Lyophilized
Quantity 1.5 mg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 5000 (WB)
Expected | apparent MW

10.97 | 12 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycine max, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot detection using anti-SUMO1 antibodies

Arabidopsis thaliana total cell extract HA-proSUMO1 (HA-epitope and pro-Small Ubiquitin like MOdifier protein1 (1), empty vector only (2), were separated on 15% gel, SDS -PAGE and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 1 000 anti-AtSUMO1 antibody (AS08 308) for 1 h, followed by incubation with 1: 15 000 secondary anti-rabbit antibodies (1h) coupled with HRP and visualization (10 seconds exposure) with standard chemiluminescent detection reganet.

Note: signal in the empty vector lane comes from endogenous SUMO1 detected by the antibody.

Additional information

Antibodies will also detect SUMO2 protein.

Related products

Related products

AS08 307A | Anti-UBQ11 | Ubiquitin, rabbit antibody (affinity purified)
AS08 307 | Anti-UBQ11 | Ubiquitin, rabbit antibody, (serum)

AS08 349 | Anti-SUMO3 | Small ubiquitin-like modifier protein 3, rabbit antibodies

Plant protein extraction buffer

Background

Background

SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.

Product citations

Selected references Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.
Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
Liu et al. (2014). SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol. 2014 Jun 3;14:153. doi: 10.1186/1471-2229-14-153.
Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.

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