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AGO set
Tag Antibody Set 

LOX2 | Lipoxygenase 2

AS08 340  |  Clonality: Monoclonal  |  Host: Mouse  |  Reactivity:  Triticum aestivum

LOX2 | Lipoxygenase 2 in the group Antibodies for Plant/Algal  / Developmental Biology / Lipid metabolism at Agrisera AB (Antibodies for research) (AS08 340)

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Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Triticum aestivum LOX2 sequence

Host Mouse
Clonality Monoclonal
Purity Affinity purified
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Glycerol 1:1 can be added to improve stability of this antibody.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 10 000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

90 kDa

Reactivity

Confirmed reactivity Triticum aestivum
Predicted reactivity Hordeum vulgare, Oryza sativa
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information LOX2 protein seems to be antigenically different compare to LOX isoforms isolated from leaves and flowers therefore this antibody being a monoclonal will not detect LOX2 from seeds. Brady et al (2014). Lipids: Structure and Function: The Biochemistry of Plants. 

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AS06 128 | anti-LOX lipoxidase rabbit antibody

Background

Background

Lipoxygenases are a family of enzymes involved in oxidative stress response. They catalyse the oxygenation of polyunsaturated fatty acids to lipidhydroperoxides. LOX’s are known to play a role in plant growth and development.

Product citations