Lysine-tRNA ligase

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AS15 2899 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana


30 st
Item No:
AS15 2899

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product information
Background Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm.

KLH-conjugated synthetic peptide derived from  Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized

100 µl

Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Photosynthetic antibody collection

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 500 (WB)
Expected | apparent MW

41 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Not reactive in
Additional information This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system.
Selected references

To be added when available, antibody released in May 2017.

Application example

western blot using anti-Lysine-tRNA ligase (At3g01060)

20 μg of protein from Arabidopsis thylakoids or chloroplasts were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated, from Agrisera ) diluted to 1: 8 000 in TTBS for 1h at RT. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 3 minute.

Total soluble proteins (stroma) extracted from 0.2 g leaf by homogenization in a buffer containing 20 mMTris-HCl, pH 9.0, 250mMNaCl, 50mMNaHCO3, 4mMMgCl2, and an EDTA-free protease inhibitor cocktail (Roche). After removal of cell debris by centrifugation for 5min at 13,000g and 4°C, a volume corresponding to 10 μg of protein was loaded on12% SDS-PAGE and blotted as descried above.

Courtesy of Dr. Rikard Fristedt, VU University Amsterdam, The Netherlands

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