H3R2me2(sym)K4me2 | Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)
AS16 3185 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
|Recommended dilution||2-5 ĩg/million cells (ChIP), 1 : 1000 (Dot), 1 : 200 (IF), 1 : 50 (IHC), 1 : 500 (WB)|
|Expected | apparent MW||
|Predicted reactivity||Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||To be added when available, antibody released in February 2016.|
Chromatin Immunoprecipitation: using anti-H3R2me2sK4me2 antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R2me2sK4me2 and 20 μl of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Dot Blot: using anti-H3R2me2sK4me2 antibodies. Lane 1: R2me2. Lane 2: R2me2/K4me2. Lane 3: K4me2. Load: 0.1, 1, 0.5, 5, and 10 picomoles of peptide. Primary antibody used at a 1:1 000 dilution for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.
Immunofluorescence: using anti-H3R2me2sK4me2 antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight® 488 secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3R2me2sK4me2 is nuclear and chromosomal. Staining: Histone H3R2me2sK4me2 is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).
Western Blot: using anti-H3R2me2sK4me2 antibodies. 30 μg of C. elegans embryo cell lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
Western Blot: using anti-H3R2me2sK4me2 antibodies. 30 μg of NIH-3T3 histone extracts. Primary antibody used at 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.
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