H3R8me2(asym) | Histone H3 (asym-dimethylated Arg8)

AS16 3177 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
H3R8me2(asym) | Histone H3 (asym-dimethylated Arg8) in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3177)


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product information
Background Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, dimethylation of H3 Arg8 (H3 R8Me2) is known as a mark of transcriptional repression.
Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid
Quantity 50 ĩg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipiation (ChIP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
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collection of antibodies to epigenetics
Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.
application information
Recommended dilution 2 ug (ChIP), 1: 100 (IF), 1: 100 (IHC), 1:500 (WB)
Expected | apparent MW

15 kDa

Confirmed reactivity Caenorhabditis elegans, Human
Predicted reactivity Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in February 2016.

application example

ChiP using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies
Chromatin Immunoprecipitation using anti-H3R8me2(asym) antibodies. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2 μg of H3R8me2(asym) and 20ul of magnetic beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR, and normalized to the input chromatin.

immunofluorescence using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies
Immunofluorescence using anti-H3R8me2(asym) antibodies. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: used at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: H3R8me2(asym) is nuclear and chromosomal. Staining: H3R8me2(asym) is expressed in green and the nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight® 594 (red).

western blot using anti-H3R8me2(asym) | Histone H3 (asym-dimethyl Arg8) polyclonal antibodies

Western Blot using antiH3R8me2(asym) antibodies. Lane 1: HeLa Histone extracts. 2. NIH-3T3 extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody diluted 1:500 overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT.

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