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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

CSD2 | Chloroplastic Cu/Zn superoxide dismutase

AS06 170 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, A.maritima, D.sanguinalis, E.crus-galli, E.tef, I.pumila, L.formosana, N.cataria, N.rtanjensis, O.sativa, P.Sogo Yukidian cultivar V3, P.vulgaris, P.sativum, Populus sp., S.superba, S.lycopersicum, S.tuberosum, Z.mays

CSD2 | Chloroplastic Cu/Zn superoxide dismutase in the group Plant/Algal Antibodies / DNA/RNA/Cell Cycle / microRNA targets at Agrisera AB (Antibodies for research) (AS06 170)

PRODUCT INFORMATION IN PDF

Qty: 
272 €
Buy 2 items of this product for 202 €/items
Buy 3 items of this product for 185 €/items

Info: Add review
product information
Background

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Immunogen

overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase

AS07 219| anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

collection of antibodies to plant stress proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

This product can be sold containing ProClin if requested.

application information
Recommended dilution 1 : 1000-1 : 4000 (WB)
Expected | apparent MW

22 | 19 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana, Armeria maritima, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Iris pumila, Liquidambar formosana, Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays
Predicted reactivity Glycne max, Pinus pinaster, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

This product can be sold containing proclin if requested.

Selected references Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.
Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19
Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64
Tuteja et al. (2015). Heterologous expression and biochemical characterization of a highly active and stable chloroplastic CuZn-superoxide dismutase from Pisum sativum. BMC Biotechnology 2015, 15:3 doi:10.1186/s12896-015-0117-0
Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.
Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.


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