AGO1 | Argonaute 1 (40 ĩg, HRP-conjugated)
AS09 527-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
116.4 | 130 kDa
|Confirmed reactivity||Arabidopsis thaliana, Nicotiana benthamiana
|Predicted reactivity||Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera|
|Not reactive in||
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
|Selected references||To be added when available, antibody released in November 2015.|
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