SE | Serrate RNA effector molecule (rabbit antibody)

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AS09 532A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana


45 st
Item No:
AS09 532A

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product information

Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).


KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana serrate protein sequence Q9ZVD0, At2g27100

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), RIP (RNA Immunoprecipitation (IP) assay), Western blot (WB)
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Collection of antibodies to micro RNA
Additional information
application information
Recommended dilution 1 : 500 (IL), 1 : 1000 (WB)
Expected | apparent MW

81 | 80 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Nicotiana benthamina, Nicotiana tabacum, Saccharum hybrid cultivar NCo 376, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Suggested blotting conditions: 8% gel, tank blotting, 200 mA/ 1h to nitrocellulose membrane.
Selected references Li et al. (2016). Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis. PLoS Genet. 2016 Nov 21;12(11):e1006422. doi: 10.1371/journal.pgen.1006422. eCollection 2016.

Application example

western blot using anti-serrate antibodies

25-30 µg of total protein from Arabidopsis thaliana rosette leaves was extracted with extraction buffer containing: 100 mM Tris HCl pH 7.5, 10 % sucrose, 5 mM EDTA, 5 mM EGTA, 300 mM NaCl, 0.75 % Triton X100, 0.15 % SDS, 1 mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) or 7.5 % nuclear fraction obtained according to the protocol from Raczyńska et al. 2014, were separated on 10 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 2.5 % milk in PBS/T for overnight at 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 250 in PBS-T for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit HRP conjugated, AS09 602, Agrisera) in 1: 5000 dlution for 1 h at RT with agitation. The blot was washed as above and developed for 5 minutes with ECL according to manufacturer's instructions. Exposure time was 600 seconds.

Courtesy of M.Sc. Mateusz Bajczyk, Department of Gene Expression, Adam Mickiewicz University, Poland

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