PR-2 | Pathogenesis-related protein 2

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AS12 2366 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Agostis stolonifera cv. ‘Penncross’Glycine max


29 st
Item No:
AS12 2366

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product information
Background PR-2 (Pathogenesis-related protein 2) is involved in the defence of plants against pathogens. This protein has a catalytic activity and is hydrolysing of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans, EC= Alternative names:Glucan endo-1,3-beta-glucosidase, acidic isoform,(1->3)-beta-glucan endohydrolase, Beta-1,3-endoglucanase, Beta-1,3-glucanase 2

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PR-2 UniProt P33157, TAIR AT3G57260

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies

AS07 208 | PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana

AS07 207
| PR-3 | CHN | class I chitinase, rabbit antibodies

AS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodies

AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies

collection of antibodies to other proteins involved in a response to pathogen attack

Plant protein extraction buffer

Secondary antibodies

Additional information This product can be sold containing Proclin if requested
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

37.3 kDa (processing aa 1-30, mature peptide 34.1 kD)

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Glycine max (roots)
Predicted reactivity Brassica juncea, Brassica oleracea, Citrus chinensis, Glycine max, Litchi chinensis, Manihot esculenta, Nicotiana tabacum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Does not cross-react with other 1,3-beta glucosidases.

Selected references Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Kim et al. (2014). The Arabidopsis Immune Adaptor SRFR1 Interacts with TCP Transcription Factors thatRedundantly Contribute to Effector-Triggered Immunity. Plant J. 2014 Apr 1. doi: 10.1111/tpj.12527.

application example

western blot using anti-PR-2 antibodies
10 µg of total protein from Arabidopsis thaliana mature leaves were extracted with 8M UREA and were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5% Milk in TBS-T 0,05% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBS-T/Milk 5% for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was seconds 60 seconds.

Courtesy of Louis-Valentin Meteignier, PhD student, University of Sherbrooke, Canada

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