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AGO set
Tag Antibody Set 

CCS | Chloroplastic copper chaperone for SOD

AS07 219 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana,  B. juncea, Z. mays, O. sativa  

CCS | Chloroplastic copper chaperone for SOD in the group Antibodies for Plant/Algal  / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS07 219)

PRODUCT INFORMATION IN PDF


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Product Information

Immunogen

The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5,  fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

33.8 | 37 kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica juncea, Oryza sativa, Zea mays
Predicted reactivity Glycne max, Solanum tuberosum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Related products

Related products

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS06 125 | anti-FeSOD | chloroplastic Fe-dependent superoxid dismutase

AS09 524 | anti-MnSOD | manganese superoxide dismutase

collection of antibodies to plant stress proteins

Background

Background

Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Product citations

Selected references Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.
Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7.  FEBS Lett. 11:2307-12.