IRT1 | Iron regulated transporter 1

AS11 1780 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana

IRT1 | Iron regulated transporter 1 in the group Plant/Algal Antibodies / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS11 1780)


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product information

IRT1 is a high afinity iron transported that plays a key role in the uptake of iron (in rhizosphere across the plasma membrane in the root epidermal layer) as well as mediates the heavy metals uptake under iron-defficiency. Is a principal regulator of iron homeaostasis in plants. Synonymes:Fe(2+) transport protein 1, Fe(II) transport protein 1.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana IRT1 sequence, Q38856, At4g19690

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. This antibody can be stored in a solution containg 50 % glycerol, final concentration. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

antibody collection for proteins involved in response to environmental stress

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

36.7 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescens, Thlaspi cerulescens
Not reactive in Horderum vulagre
Additional information
Selected references Selote et al. (2014). Iron-binding E3 ligase mediates iron response in plants by targeting bHLH transcription factors. Plant Physiol. 2014 Dec 1. pii: pp.114.250837.
Ivanov et al. (2014). SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1. Plant Cell. 2014 Mar 4.

application example


western blot detection using anti-IRT1 antibody

5 µg of total protein from Arabidopsis thaliana wild type (Col-0) and IRT1 mutant (irt1-1) extracted with SDG buffer (62 mM Tris-HCL pH 8.6, 2.5 % SDS, 2 % dithiothreitol, 10 % glycerol) were separated on 15 % SDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked with 5 % milk in TBST for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation in TBST with 2.5 % milk. The antibody solution was decanted and the blot was rinsed briefly three times, then washed once for 10 min in TBST at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 3 seconds.

Iron-sufficient medium contained 50 µM Fe, +Fe condition, iron-deficient medium 0 µM Fe, -Fe condition.

Courtesy Dr. Petra Bauer and Dr. Rumen Ivanov, Saarland University, Germany

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