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ZCP1 | Zinc Chaperone Protein

AS14 2770  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

ZCP1 | Zinc Chaperone Protein in the group Plant/Algal Antibodies / Environmental Stress / Heavy metal stress at Agrisera AB (Antibodies for research) (AS14 2770)

PRODUCT INFORMATION IN PDF

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Antibody available in 2016. For more information please inquire.
product information
Background ZCP1 belongs to a large family of putative metallochaperones, likned with maintainance of zinc homeostasis in all living organisms.
Immunogen

Recombinant, full length ZCP1 protein of Chlamydomonas reinhardtii, Cre14.g630871, UniProt: A0A059VIM6

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS12 1848 | anti-ZCP2 | Zinc Chaperone Protein, rabbit antibodies
collection of antibodies to various Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

35 | 46 kDa

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Emiliania huxleyi
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
Selected references to be added when available, antibody released in January 2015

application example

 

western blot using anti-ZCP1 antibodies

Chlamydomonas reinhardtii
whole-cell extracts corresponding to 10 μl 1 x 10 7 cells/ml per well (except dilutions as indicated) were separated on a 12% SDS-PAGE gel and blotted to nitrocellulosefor 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% bovine calf serum in PBS-T overnight at 4deg C. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech) diluted to 1:3000 in PBS-T for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T, then rinsed with TBS, and developed.

Courtesy of Crysten Blaby, UCLA, USA

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