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Gamma-glutamyl-cysteine

AS11 1811 | Clonality: Polyclonal | Host: Rabbit | Reactivity:A.thalina, C. pepo, N. tabacum

Gamma-glutamyl-cysteine in the group Plant/Algal Antibodies / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS11 1811)

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product information
Background

γ-glutamyl-cysteine is a precursor of glutathione and is synthesized by linkage of glutamate and cysteine by γ-glutamylcysteine synthetase. Synonymes:gamma-glutamyl-cysteine, γ-glutamyl-cysteine. Alternative names: γ-EC, γ-Glu-Cys.

Immunogen

γ-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 2x100 µg
Reconstitution For reconstitution add 100 µl of sterile water per tube.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunocytochemistry (ICC), Immunogold (IG)
Related products AS06 186 | Anti-Gamma-ECS | Gamma glutamylcysteine synthase, rabbit antibody

other antibodies to proteins involved in response to heavy metals

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 50 (ICC), (IG)
Expected | apparent MW
Confirmed reactivity Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Immunogold labeling for electron microscopy

Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Selected references Koffler et al. (2011). Subcellular distribution of glutathione precusors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 53: 930-941.


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