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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

Gamma-ECS | Gamma glutamylcysteine synthase

AS06 186  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: N.tabacum, S. lycopersicum, Z. mays

Gamma-ECS | Gamma glutamylcysteine synthase in the group Plant/Algal Antibodies / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS06 186)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Add review
product information
Background

Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays  gamma-ECS Q8W4W3

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Related products AS11 1811 | Anti-Gamma-glutamyl-cysteine, rabbit antibodies

collection of antibodies to plant stress proteins

Additional information Total IgG concentration is 5.2 µg/ µl.
application information
Recommended dilution 1 : 5000 (IL), 1 : 5000 (WB)
Expected | apparent MW

50 kDa

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabcum, Solanum lycopersicum, Zea mays
Predicted reactivity Pisum sativum, Riciuns communis, Oryza sativa, Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Antibodies has been used in immunolocalization on Arabidopsis thaliana.

Selected references Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com