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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

GR | Glutathione reductase

AS06 181  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.toxicaria, B.rapa, N. tabacum, M. sativa, O. sativa, S. vulgaris, S. tuberosum, Z. mays

GR | Glutathione reductase in the group Plant/Algal Antibodies / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS06 181)

PRODUCT INFORMATION IN PDF

Qty: 
360 €

Info: Read reviews
product information
Background

Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for  plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).

Immunogen

Maltose binding protein (MBP) fusion of Zea mays GR, O64409

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Immunoprecipitation (IP), Western blot (WB)
Related products

AS06 183 | anti-GS, glutathione synthase antibodies

collection of antibodies to stress proteins

Additional information

Total IgG concentration is 7 µg/ µl

application information
Recommended dilution 2 µg (IP), 1 : 1000 (IL), 1 : 5000 (WB)
Expected | apparent MW

54 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Silene vulgaris, Solanum tuberosum, Zea mays, Scenedesmus quadricauda (algae)
Predicted reactivity Brassica rapa, Glycine max, Oryza sativa, Populus balsamifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody will recognize the chloroplastic and cytoplasmic forms of the enzyme.

Selected references Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.
Hattab et al. (2015). Characterisation of lead-induced stress molecular biomarkers in Medicago sativa plants. Environm. Exp. Botany. Volume 123, March 2016, Pages 1–12.
Shaw et al. (2015). β-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29.
Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
>Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)
Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.

application example

10 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Nicotiana tabaccum leaf extracted with PEB, (3) Zea mays extracted with PEB, (4) Hordeum vulgare leaf extracted with PEB, (5) Physcomitrella patens total cell extracted with PEB, (6) Chlamydomonas reinhardtii total cell extracted with PEB,  (7) Synochocystis elongatus total cell extracted with PEB, extracted with PEB, were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked in 2 % low fat dry milk in TBS-T (0.1 % Tween 20) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:30 000 for 1h at room temperature with agitation. The blots were washed as above and developed for  30 seconds  with WEST PICO reagent according the manufacturers instructions.

The nature of 40 kDa cross reaction in this experiment is not known.

 

western blot using anti-GR antibodies
 

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