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3-nitroY | Nitrotyrosine

AS10 706-100  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: nitrotyrosine

3-nitroY | Nitrotyrosine  in the group Plant/Algal Antibodies / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 706-100)

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product information
Background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.

Immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid

Host Mouse
Clonality Monoclonal
Clone IgG2A
Purity Protein G purified, in PBS with 50 % glycerol, 0.09% sodium azide.
Format Liquid
Quantity 100 µg
Reconstitution
Storage Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Flowcyt (Flow cytometry), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Related products

AS10 706-25 | anti-nitrotyrosine mouse antibody, 25 µg

Secondary antibodies

Additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4,  0.09 % sodium azide, 50 % glycerol

application information
Recommended dilution 1: 100 (IHC), 1: 1400 (WB). The exact and optimal working dilution should be determined by the investigator.
Expected | apparent MW
Confirmed reactivity Dog, Human, Mouse, Rat
Predicted reactivity Higher plants
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
with non-nitrated tyrosine. Not species specific.

0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.

Antibody works paraffin-embedded sections. 

 

Selected references Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.

Application example

IHC using nitrotyrosine antibodies IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.

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