3-nitroY | Nitrotyrosine
AS10 706-100 | Clonality: monoclonal | Host: Mouse | Reactivity: nitrotyrosine
|Recommended dilution||1: 100 (IHC), 1: 1400 (WB). The exact and optimal working dilution should be determined by the investigator.|
|Expected | apparent MW|
|Confirmed reactivity||Dog, Human, Mouse, Rat|
|Predicted reactivity||Higher plants|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.
|Selected references||Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:
Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.
IHC using anti-Nitrotyrosine mouse monoclonal antibodies, Clone 39B6. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Anti-Nitrotyrosine mouse monoclonal antibody Dilution: 1:1000000 for 12 h/4°C. Secondary Antibody: goat anti-Mouse biotin conjugated at 1:2000 1 h/RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at firstname.lastname@example.org