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ACD | alpha crystalline domain of sHSP

AS15 3029  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: recombinant ACD domain od sHSP

ACD | alpha crystalline domain of sHSP in the group Plant/Algal Antibodies / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS15 3029)

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product information
Background ACD (alpha crystalline domain)
Immunogen KLH-conjugated peptide derived from sequences of ACD domain
Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS08 350 | anti-DnaK2 | heat shock protein 70-2 (HSP70-2)

collection of antibodies to heat shock proteins

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

16.6 kDa

Confirmed reactivity recombinant sHSP with ACD domain
Predicted reactivity ACD domain of sHSP
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in May 2016.

Application example

 

 

 Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis  PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody  (1: 10 000).

 

 Western blot using anti-Hsp16.6 antibodies

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