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AGO set
Tag Antibody Set 

HSF1 | Heat shock factor 1

AS06 178  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomons reinhardtii

HSF1 | Heat shock factor 1 in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS06 178)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

C-terminal part of Chlamydomonas reinhardtii HSF1 (Exons 7 to 9), UniProt: A1YTN3

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1. 3000 (WB)
Expected | apparent MW

78.4 | 100 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Chlamydomonas reinhardtii
Not reactive in Higher plants

Application examples

Additional information

Unspecific cross-reactions at 40-50 kDa.

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Background

Background

HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.

Product citations

Selected references Schulz-Raffelt et al. (2007).. Heat shock factor 1 is a key regulator of the stress response in Chlamydomonas. The Plant Journal  52: 286-295

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