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Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70

AS07 233  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Triticum sp., Pisum sativum 

HSC70/HSP70 | Heat shock cognate protein 70 / heat shock protein 70 in the group Plant/Algal Antibodies / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS07 233)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Add review
product information
Background

The heat shock cognate protein 70 (HSC70) is a constitutively expressed house keeping protein which can act as a molecular chaperone towards other proteins.

Immunogen

HSC70 purified from wheat germ by ATP affinity chromatography 

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
Related products AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic, rabbit antibodies AS08 348 | anti-HSP70 | heat shock protein 70, chloroplastic , rabbit antibodies
AS08 347 | anti-HSP70 | heat shock protein 70, mitochondrial, rabbit antibodies

AS05 086 | anti-UGPase (cytoplasm marker), rabbit antibodies

Collection of antibodies to heat shock proteins

Additional information

This antibody will not react with bip or CSS1. Tested on 2D gels antibody reactos strongly with two different proteins, hsc70 and hsp70.

application information
Recommended dilution 1 : 50 000 (WB)
Expected | apparent MW

70 kDa

Confirmed reactivity Cucurbita pepo, Pisum sativum, Triticum sp.
Predicted reactivity Dicots, Monocots
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

This antibody can inhibit protein import into organelles in vitro.

Selected references Crookes & Olsen (1998), The effects of chaperones and the influence of protein assembly on peroxisomal protein import. J. Biol. Chem. 27:17236-17242


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