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AGO set
Tag Antibody Set 

HSP17.6 | Cytosolic class I heat shock protein 17.6 (chicken antibody)

AS08 284 | Clonality: Polyclonal | Host: Hen | Reactivity: Arabidopsis thaliana

HSP17.6 | Cytosolic class I heat shock protein 17.6 (chicken antibody) in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 284)

PRODUCT INFORMATION IN PDF

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355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) P13853, At1g53540

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Liquid in PBS pH 8.0, 0.02% sodium azide
Quantity 200 µl
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17.6 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

 

10 µg of total protein from (C) Arabidopsis thaliana WT, non-stressed, (HS) Arabidopsis thaliana WT stressed at 38ºC were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000).

 

western blot using anti-Hsp17.6 antibodies

Additional information

Additional information Total IgY concentration is 32.2 µg/µl.

There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.

Related products

Related products

AS07 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

Background

Background

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Product citations

Selected references Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.