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AGO set
Tag Antibody Set 

HSP101 | ClpB heat shock protein, C-terminal

AS08 287 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequilana, Citrus sp., C. sativus

HSP101 | ClpB heat shock protein, C-terminal in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS08 287)

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Datasheet Product citations Customer reviews

Product Information

Immunogen

Recombinant protein of Arabidopsis thaliana Hsp101/ClpB, C-terminal 145 amino acids P42730, At1g74310

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

101 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak
Predicted reactivity Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

When testing a new species with this antibody perform a following experiment:
prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample.
Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.

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collection of antibodies to plant heat shock proteins

Background

Background

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.

Product citations

Selected references Balfagón et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029.
Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.
Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.