HSP17.6 | Cytosolic class I heat shock protein 17.6 (rabbit antibody)
AS07 254 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequilana, Citrus sp., C. sativus, I. pumilla (perennial monocot), P. ternata, P. sylvestris, S. vulgaris, V. faba
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15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
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