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AGO set
Tag Antibody Set 

HSP17.6 | Cytosolic class I heat shock protein 17.6 (rabbit antibody)

AS07 254 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, A. tequilana, Citrus sp., C. sativus, I. pumilla (perennial monocot), P. ternata,  P. sylvestris, S. vulgaris, V. faba

HSP17.6 | Cytosolic class I heat shock protein 17.6 (rabbit antibody) in the group Antibodies for Plant/Algal  / Environmental Stress / Heat shock at Agrisera AB (Antibodies for research) (AS07 254)

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Datasheet Product citations Add review

Product Information

Immunogen

Recombinant protein. Arabidopsis thaliana Hsp17.6 CI (class one) P13853, At1g53540

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

17.6 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Agave tequilana var. Weber, Citrus sp., Cucumis sativus, Iris pumilla (perennial monocot), Pinellia ternata, Pinus sylvestris, Silene vulgaris, Solanum tuberosum, Vicia faba
Predicted reactivity Higher plants, Nicotiana tabacum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-Hsp17.6 antibodies

15 µg of total protein from (HS) heat shocked Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.6 were separated on 15%SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000).

Additional information

There are six total class I genes. Essentially this antibody might react to some extent with all of them. But does not react with class II, organelle, or any other shsp classes.

Related products

Related products

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

Background

Background

Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.

Product citations

Selected references Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018.
Balfagón et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018
Pantelić et al. (2018). Effects of high temperature on in vitro tuberization and accumulation of stress-responsive proteins in potato. Hortic. Environ. Biotechnol. (2018) 59: 315.
Zhu et al. (2018). Cloning and expression of a new cytoplasmic small heat shock protein gene from Pinellia ternata. Acta Physiologiae Plantarum March 2018, 40:44.
Murano et al. (2017). A purine-type heat shock protein 90 inhibitor promotes the heat shock response in Arabidopsis. Plant Biotechnology Reports April 2017, Volume 11, Issue 2, pp 107–113.
Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]
Yamauchi et al. (2015). Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression.
Pyatrikas et al. (2014). Mitochondrial Retrograde Regulation of HSP101 Expression in Arabidopsis thaliana under Heat Stress and Amiodarone Action. Russian J. Plant Physiol. 61 (1):88-98. (Western blot, cell culture)
Florentin et al. (2013). Stress induces plant somatic cells to acquire some features of stem cells accompanied by selective chromatin reorganization. Dev. Dyn. Oct; 242(10):1121-33.