HSP18.5 | class IV heat shock protein

355 €

AS11 1628   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity:Arabidopsis thaliana


28 st
Item No:
AS11 1628

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product information

HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein


recombinant Arabidopsis thaliana Hsp18.5 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Plant protein extraction buffer

Secondary antibodies

Additional information

As hsp18.5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature. Normally I heat stress the seedling on a sealed agar plate for 2 hours. This assures that the humidity around the plant is very high. Low humidity can allow the plant to cool down through transpiration.  If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment. Heat stressing plants in microphage tubes does not work that well.

application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

18.5 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.

Please, note that longer transfer time might result in losing a signal for this protein.

Selected references

to be added when available.

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