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Tag Antibody Set 

PFOR | pyruvate oxidoreductase

AS07 275 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

PFOR | pyruvate oxidoreductase in the group Antibodies for Plant/Algal  / Fermentation at Agrisera AB (Antibodies for research) (AS07 275)

PRODUCT INFORMATION IN PDF

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381 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide conserved in Chlamydomonas reinhardtii PFOR protein UniProt: A8JEH2

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

130 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity Volvox carteri
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

Chlamydomonas reinhardtii total cell extract 15 µg/lane from cells acclimated to dark anoxia following  (1) 2 h; (2) 4h; (3) 8 h of anoxia imposition. Total proteins were extracted with 50 mM Tris buffer (pH 8.0) containing 10 mM EDTA and 2% SDS,  and were separated by SDS-PAGE using a 10% polyacrylamide gel  and then transferred 1h at RT to PVDF membranes. The membranes were blocked with a 5% milk in TBS-T for 2 h. Blot was incubated in the primary anti-PFOR antibody at a dilution of 1: 1 000 over night at 4°C with agitation in 3 % milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescence detection reagent, according to the manufacturers instructions. Exposure time was 5 seconds.

Courtesty Dr. Caludia Catalanotti, Carnegie Institution, USA

 
 western blot detection of PFOR in Chlamydomonas

 


Additional information

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Background

Background PFOR (pyruvate-ferredoxin oxidoreductase) is an enzyme which belongs to a family of oxidoreducatases and participates in 4 metabolic pathways: pyruvate metabolism, propanoate metabolism, butanoate metabolism and reductive carboxylate cycle (carbon dioxide fication). Alternative names: pyruvate oxidoreductase, pyruvate synthetase, pyruvic-ferredoxin oxidoreductase.

Product citations

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