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PetC | Rieske iron-sulfur protein of Cyt b6/f complex

AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Thalassiosira guillardii, Z. mays

PetC | Rieske iron-sulfur protein of Cyt b6/f complex  in the group Plant/Algal Antibodies / Global Antibodies at Agrisera AB (Antibodies for research) (AS08 330)

PRODUCT INFORMATION IN PDF

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product information
Background

Rieske Iron-Sulfur Protein (Q9ZR03)is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1

Immunogen

KLH-conjugated synthetic peptide which shows strong conservation across higher plants including Arabidopsis thaliana Q9ZR03, At4g03280, Chlamydomonas reinhardtii P49728 and Synechococcus sp. Q5N5B0

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water-
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS08 330S | PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard for quantitation of PetC protein

AS07 230 | anti-PetC, Rieske iron-sulfur protein of Cyt b6/f complex

Additional information

This product can be sold containing Proclin if requested.

application information
Recommended dilution 1 : 5000-1 : 10 000 (WB)
Expected | apparent MW

23 kDa

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Echinola crus-galli, Euglena sp., Haematococcus pluvialis, Nicotiana tabacum, Panicum miliaceum, Pisum sativum, Spinacia oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Zea mays
Predicted reactivity
Acetabularia acetabulum, Brachypodium distachyon, cyanobacteria, Calothrix sp. PCC 7507, Catalpa bungei, Cicer arietinum, Crocosphaera watsonii, Cynodon dactylon, Gossypium raimondii , Hordeum vulgare, Lyngbya aestuarii,Microcystis aeruginosa, Nicotiana tabacum, Pisum sativum, Ricinus communis , Saccharum hybrid cultivar ROC22, Selaginella moellendorffii , Sorghum bicolor, Oryza sativa,  Physcomitrella patens, Phormidesmis priestleyi, Populus trichocarpa, Sonneratia alba, Triticum aestivum, Zostera marina, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Wood et al. (2018). Dynamic thylakoid stacking regulates the balance between linear and cyclic photosynthetic electron transfer. Nat Plants. 2018 Feb;4(2):116-127. doi: 10.1038/s41477-017-0092-7. Xing et al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017.
Zang et al. (2017). Characterization of the sulfur-formation (suf) genes in Synechocystis sp. PCC 6803 under photoautotrophic and heterotrophic growth conditions. Planta. 2017 Jul 14. doi: 10.1007/s00425-017-2738-0.
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
Zhang et al. (2016). A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis. Biotechnol Bioeng. 2016 Oct;113(10):2088-99. doi: 10.1002/bit.25976. Epub 2016 Mar 28.

application example

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Euglena sp. extracted with PEB, (3) Synechococcus elongatus whole cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

 


AS08 300 western.jpg

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