BiP | Lumenal-binding protein (100 ĩg)

AS09 481-100| Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, C. sativus, R. sativa L. Tokinashi-daikon, S. oleracea, S. lycopersicum, S. tuberosum, T. aestivum, Z. mays, O. europaea, P. abies, P. patens, Ch. reinhardtii

BiP | Lumenal-binding protein (100 ĩg) in the group Plant/Algal Antibodies / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 481-100)


407 €

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product information

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540  Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080  Q8H1B3

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 2 x 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water to each tube.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Related products

AS09 615 | BiP2 | lumenal-binding protein 2, goat antibody
AS09 614 | BiP2 | lumenal-binding protein 2, hen antibody
AS09 481PRE | BiP | lumenal-binding protein, pre-immune serum
antibodies to plant endomembrane system proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Method for plant ER isolation is available here.

application information
Recommended dilution 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Expected | apparent MW

73.5 | 80 kDa

Confirmed reactivity Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtii, Cucumis sativus, Nicotiana benthamiana, Olea europaea, Picea abies, Physcomitrella patens, Raphanus sativa L. Tokinashi-daikon, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zeay mays
Predicted reactivity Capsella rubella, Vitis vinifera, Ricinus comminus, Glycine max. Nicotiana tabacum, Hordeum vulgare, Oryza sativa, Picea sitcHensis, Populus trichocarpa.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel. This antibody has so far not worked in IP.

Selected references Hu et al. (2015). Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Jan 12. pii: pp.114.252023.
Ivanov et al. (2014). SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1. Plant Cell. 2014 Mar 4.
Bommert et al. (2013). The maize Gα gene COMPACT PLANT2 functions in CLAVATA signalling to control shoot meristem size. Nature,Sep 11. doi: 10.1038/nature12583. (western blot, Zea mays)
Sulimanet al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization).

application example western blot

western blot using plant anti-BiP antibodies

5 µg of total protein from A.thaliana (1), H. vulgare (2)P. sativum (3)*Z. mays (4)C. sativus(5)S. tuberosum (6)S. oleracea (7)S. lycopersicum (8) P. patens (9)*Ch. reinhardtii (10)  extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from  Agrisera AS09 602) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds. * Lack of the signal or its low signal intensity in those samples can be due to the sample biology. If you work with those species, please inquire.

application example immunolocalization

 Immunofluorescence using plant anti-BiP antibody

BiP localization in 5 days old Arabidopsis thaliana roots (A), 3 days old Triticum aestivum roots (B).
BiP signal shown in red, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-BiP primary antibody diluted in 1: 600 and ALEXA 555 conjugated anti-rabbit secondary antibody (red color) have been used. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University

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