BiP | Lumenal-binding protein (chicken antibody)

AS09 614 | Clonality: Polyclonal | Host: Hen | Reactivity: Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays

BiP | Lumenal-binding protein (chicken antibody) in the group Plant/Algal Antibodies / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 614)


381 €

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product information

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540  Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080  Q8H1B3

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY in PBS pH 7.4
Format Liquid
Quantity 100 ĩg
Storage Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications immunofluorescence (IF), Western blot (WB)
Related products

AS09 481 | BiP2 | lumenal-binding protein 2, rabbit antibody

AS09 615 | BiP2 | lumenal-binding protein 2, goat antibody

antibodies to plant endomembrane system proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibody solution contains 0.02% sodium azide as preservative

Method for plant ER isolation is available here.

application information
Recommended dilution 1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Expected | apparent MW

73.5 | 80 kDa

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Physcomitrella patens, Spinacia oleracea, Zea mays
Predicted reactivity Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Selected references Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.

application example



5 µg of total protein from A.thaliana (1), H. vulgare (2) Z.mays (3) S. oleracea (4), extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgY horse radish peroxidase conjugated, from  Agrisera AS09 603) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds.



western blot detection of plant BiP using hen antibody


immunolocalization of plant BiP using hen antibody


BiP localization in 5 days old Arabidopsis thaliana roots. BiP signal shown in green, DAPI in blue. The material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Chicken anti-BiP primary antibody was diluted in 1: 1000 and DyLight®488 conjugated goat anti-chicken secondary antibody AS09 622 (green color) was diluted in 1: 1000. Co-staining with DAPI visualized nucleus (blue color).  Scale bar – 10 µm.

Courtesy Dr. Taras Pasternak, Freiburg University


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