V-ATPase, H | vacuolar H+-ATPase subunit H

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AS09 499 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


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AS09 499

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product information

V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit H is part of the peripheral V1 complex. Alternative name: vacuolar proton pump subunit H, vacuolar H(+)-ATPase subunit H


KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit H Q9LX65, At3g42050

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

collection of antibodies to vacuolar proteins

AS09 466 | anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a

AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPase

AS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit beta

AS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa)

ASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit D

AS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPase

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol of isolation of vacuolar membranes can be found here.

application information
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

50 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Picea sitcHensis, Populus balsamifera subsp. trichocarpa, Spinacia oleracea
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

to be added when available

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