TIP2 | Tonoplast intrinsic protein 2;1, 2;2, 2;3

375 €

AS12 2619 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


14 st
Item No:
AS12 2619

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product information

TIP2  (Aquaporin TIP) is a protein required for the transport of water from the vacuolar compartment to the cytoplasm. Alternative names: Delta-tonoplast intrinsic protein, Delta-TIP.


KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana TIP2;1 (100 %) UniProt:Q41951, TAIR:AT3G16240, TIP2;2 (90%) UniProt: Q41975, TAIR:AT4G17340, TIP2;3 (81%) UniProt: Q9FGL2, TAIR: AT5G47450

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to tonoplast proteins

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

Plant protein extraction buffer

Secondary antibodies

Additional information Protocol for isolation of plant vacuolar membranes can be found here.
application information
Recommended dilution 1 : 1000 -1 : 2000 (WB)
Expected | apparent MW

25.6 | 24 kDa (Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Antirrhinum majus, Brassica oleracea, Chorispora exscapa, Gossypium mexicanum, Helianthus annuus, Medicago truncatula, Malus prunifolia, Nicotiana tabacum, Populus balsamifera subsp. trichocarpa, Raphanus sativus, Ricinus communis, Solanum nigrum , Solanum tuberosum, Vitis vinifera, Quercus petraea
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Selected references

to be added when available, antibody releases in January 2014

application example

10 µg of total proteinfrom Arabidopsis thaliana roots (Ecotype-Columbia) were separated on 12%SDS-PAGE and blotted 1h to PVDF membrane. Blots were blocked with 5%low-fat milk powderfor 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 over night with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T (0.05% Triton X.100)at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidaseconjugated from donkey diluted to 1:15 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL PlusTM (GE Healthcare Life Sciences, Amersham, UK) according to the manufacturer's instructions. Exposure time was 5 seconds.

Courtesy of Dr. Azeez Suliman Beebo, University of Gothenburg, Sweden

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