Name:
Phone:
E-mail:
Message
AGO set
Tag Antibody Set 

Toc159 | Chloroplast outer envelope membrane translocon complex protein

AS07 239  |  Clonality: Polyclonal  |  Host: Guinea pig  |  Reactivity:  Pisum sativum

Toc159 | Chloroplast outer envelope membrane translocon complex protein in the group Antibodies for Plant/Algal  / Photosynthesis  / Chaperones/Transporters at Agrisera AB (Antibodies for research) (AS07 239)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Add review

Product Information

Immunogen

Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)

Host Guinea pig
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 500 (CM), 1 : 10 000 (WB)
Expected | apparent MW

150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx. 29 kDa degradation product (cross reaction with GTPase domain)

Reactivity

Confirmed reactivity Pisum sativum
Predicted reactivity

Dicots, Oryza sativa

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Additional information

Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy.

Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance. 

Related products

Related products

AS06 150  |  anti-Toc75 (monocots and dicots)

AS08 292  |  anti-Toc75 (Pisum sativum)

Background

Background

Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.

Product citations

Selected references Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009.