Agrisera Super Deal

Primary/Secondary/ECL

Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright, for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

Toc34 | Chloroplast outer envelope membrane translocon complex protein (GTPase domain)

AS07 238 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii

PRODUCT INFORMATION IN PDF

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product information
Background		Toc34 (tranlocon at the outer envelope membrane of chloroplasts) is located in the outer chloroplast membrane and functions as GTP dependent receptor protein translocation inside the chloroplast.
Immunogen		gel piece with purified GTPase domain of Arabidopsis thaliana that is missing the transmembrane domain with a His-tag on the C-terminus
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS07 211 \| anti-Toc34 \| OEP34, chloroplast outer envelope membrane translocon complex protein AS07 235 \| anti-Toc34 \| OEP34, chloroplast outer envelope membrane translocon complex protein AS07 237 \| anti-Toc34 \| OEP34, chloroplast outer envelope membrane translocon complex protein AS08 345 \| anti-Toc75 \| POTRA domain 1 AS08 351 \| anti-Toc75 \| POTRA domain 3 Secondary antibodies Plant protein extraction buffer
Additional information		Does not cross- react with closely related protein Toc33

application information
Recommended dilution		1 : 10 000 (WB)
Expected \| apparent MW		34 \| 27 kDa
Confirmed reactivity		Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity		Dicots
Not reactive in		Nicotiana tabacum
Additional information
Selected references		Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060. Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com