Toc34 | Chloroplast outer envelope membrane translocon complex protein (GTPase domain)
AS07 238 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Chalmydomonas reinhardtii
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Product Information
Immunogen
gel piece with purified GTPase domain of Arabidopsis thaliana that is missing the transmembrane domain with a His-tag on the C-terminus
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 10 000 (WB)
Expected | apparent MW
34 | 27 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity
Dicots
Not reactive in
Nicotiana tabacum
Application examples
Additional information
Additional information
Does not cross- react with closely related protein Toc33
Background
Background
Toc34 (tranlocon at the outer envelope membrane of chloroplasts) is located in the outer chloroplast membrane and functions as GTP dependent receptor protein translocation inside the chloroplast.
Product citations
Selected references
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060.
Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.
Warakanont et al. (2015). Chloroplast lipid transfer processes in Chlamydomonas reinhardtii involving a TRIGALACTOSYLDIACYLGLYCEROL 2 (TGD2) orthologue. Plant J. 2015 Oct 23. doi: 10.1111/tpj.13060.
Formighieri et al. (2012). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of Tail-anchored proteins. Plant J. Nov. 20.
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