Agrisera Super Deal

Primary/Secondary/ECL

Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright, for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

RbcS | Rubisco small subunit (SSU)

AS07 259 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, C. sativus, H. vulgare, M. domestica, N. tabacum, S. lycopersicum

PRODUCT INFORMATION IN PDF

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product information
Background		Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.
Immunogen		KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1A UniProt: P10795,TAIR: AT1G67090, and 1B of Arabidopsis thaliana UniProt: P10796 At5g38430
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS07 259A \| anti-RbcS \| Rubisco small subunit (SSU) (affinity purified) rabbit antibodies AS03 037 \| anti-RbcL \| Rubisco large subunit, form I and form II (50 µl), rabbit antibodies AS03 037A \| anti-RbcL \| Rubisco large subunit, form I and form II (50 µg affinity purified), rabbit antibodies AS03 037-HRP\| anti-RbcL \| Rubisco large subunit, form I and form II (40 µg, HRP-conjugated), rabbit antibodies AS15 2955 \| anti-RbcL II \| Rubisco large subunit, form II (50 µl), rabbit antibodies AS15 2955S \| RbcL II \| Rubisco form II positive control/quantitation standard AS01 017 \| anti-RbcL \| Rubisco large subunit, form I, chicken antibodies AS01 017S \| Rubisco protein standard for quantitative western blot or positive control AS03 037PRE \| Rubisco large subunit, pre-immune serum AS09 409 \| Rubisco quantitation kit AS15 2994 \| Rubisco ELISA quantitation kit AS07 218 \| anti-Rubisco \| 557 kDa hexadecamer, rabbit antibody to a whole protein AS07 222 \| anti-RbcS \| Rubisco small subunit (SSU) from pea, rabbit antibodies matching secondary antibody
Additional information

application information
Recommended dilution		1 : 5000 (WB)
Expected \| apparent MW		20 \| 15 kDa
Confirmed reactivity		Arabidopsis thaliana, Chlamydomonas reinhardtii, Cucumis sativus, Hordeum vulgare, Malus domestica, Nicotiana tabacum
Predicted reactivity		Algae, Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Marchantia paleacea, Musa acuminata, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea mays
Not reactive in		Cyanobacteria
Additional information		This product can be sold containing ProClin if requested.
Selected references		Ravi et al. (2018). Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. Int J Mol Sci. 2018 Feb 16;19(2). pii: E585. doi: 10.3390/ijms19020585. Hartings et al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401. Yin et al. (2016). Interplay between mitogen-activated protein kinase and nitric oxide in brassinosteroid-induced pesticide metabolism in Solanum lycopersicum. J Hazard Mater. 2016 Oct 5;316:221-31. doi: 10.1016/j.jhazmat.2016.04.070. Epub 2016 Apr 29. Robert et al. (2015). Leaf proteome rebalancing in Nicotiana benthamiana for upstream enrichment of a transiently expressed recombinant protein. Plant Biotechnol J. 2015 Aug 19. doi: 10.1111/pbi.12452. Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479. Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18. Huang et al. (2015). Rubisco accumulation is important for the greening of the fln2-4 mutant in Arabidopsis. Volume 236, July 2015, Pages 185–194. Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843. Sun et al. (2014). The response of rbcL, rbcS and rca genes in cucumber, (Cucumis sativus L.) to growth and induction light intensity. Acta Physiol Plant, October 2014, Volume 36, Issue 10, pp 2779-2791

Application example

2 µg of total protein from Arabidopsis thaliana (1), Hordeum vulgare (2), extracted with Agrisera PEB extraction buffer (AS08 300) Samples were diluted with 1X sample buffer (NuPAGE LDS sample buffer (Invitrogen) supplemented with 50 mM DTT and heat at 70°C for 5 min and keept on ice before loading. Protein samples were separated on 4-12% Bolt Plus gels, LDS-PAGE and blotted for 70 minutes to PVDF using tank transfer. Blots were blocked immediately following transfer in 2% blocking reagent (GE RPN 2125; Healthcare) or 5% non-fat milk dissolved in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 (in blocking reagent) for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, and then washed 1x15 min and 3x5 min with TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602, Agrisera) diluted to 1:25 000 in blocking reagent for 1h at room temperature with agitation. The blots were washed as above. The blot was developed for 5 min with TMA-6 (Lumigen) detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (VersaDoc MP 4000) and Quantity One software (Bio-Rad). Exposure time was 25 seconds.

Courtesy of Mayura Manerkar, Mount Alison University, Canada

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