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AGO set
Tag Antibody Set 

AtpA | Alpha subunit of ATP synthase, chloroplastic

AS08 304  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. modesticaldum, Z. mays

AtpA | Alpha subunit of ATP synthase, chloroplastic in the group Antibodies for Plant/Algal  / Photosynthesis  / ATP synthase at Agrisera AB (Antibodies for research) (AS08 304)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

Recombinant maize chloroplast AtpA P05022

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 200 (IG), 1 : 5 000 (WB)
Expected | apparent MW

55 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Begonia sp., Capsicum annuum, Heliobacterium modesticaldum, Zea mays
Predicted reactivity Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Cyanobacteria
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

Western blot
 
Lane 1: Arabidopsis thaliana total leaf protein extract;
Lane 2: Zea mays total leaf protein extract
5 µg of total protein were loaded per each lane.
 
Experimental conditions:
 
Proteins were separated in a 12% or 5-15% gradient gel following by a transfer to nitrocellulose membrane. Membrane was blocked with TBST + 4% non-fat dried milk, 20 min following by three washes in TBST. Incubation time with primary and secondary antibodies was 1 hr primary, 30 min for secondary antibodies. Manufacturer of secondary antibodies: Bio-Rad
AS08 304 western blot picture.jpg

Additional information

Additional information

Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757

Related products

Related products

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS08 312 | anti-ATP synthase subunit gamma antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS08 370 | anti-ATP synthase whole enzyme

Background

Background

ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.

Product citations

Selected references Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)
Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.
Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.
Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.
Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.
Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.

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