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AtpC | Gamma subunit of ATP synthase, chloroplastic

AS08 312  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii

AtpC | Gamma subunit of ATP synthase, chloroplastic in the group Plant/Algal Antibodies / Photosynthesis  / ATP synthase at Agrisera AB (Antibodies for research) (AS08 312)

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product information
Background

ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS08 370 | anti-ATP synthase, whole enzyme

AS08 304 | anti-ATP synthase subunit alpha antibody

AS03 030 | anti-ATP synthase subunit beta hen antibody

AS05 071 | anti-ATP synthase subunit c antibody

AS07 260 | H+ATPase | plasma membrane H+ATPase

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 1000 (ELISA), 1: 10 000 (WB)

Expected | apparent MW

35.3 | 42 (Chlamydomonas reinhardtii)

35.6 | 38 (Spinacia oleracea)

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii
Predicted reactivity

Arachis hypogaea, Auxenochlorella protothecoides, Bathycoccus prasinos, Cephalotus follicularis, Cicer arietinum, Coccomyxa subellipsoidea (strain C-169), Cucumis melo, Cyanobacteria, Cynara cardunculus var. scolymus, Gossypium hirsutum, Klebsormidium flaccidum, Glycine max, Gossypium hirsutum, Helianthus annuus, Lens culinaris, Micromonas pusilla (strain CCMP1545), Monoraphidium neglectum, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Ostreococcus tauri, Phaseolus vulgaris, Physcomitrella patens, Pisum sativum, Populus jackii, Populus trichocarpa, Ricinus communis, Tetraselmis sp. GSL018, Theobroma cacao, Vigna unguiculata, Vitis vinifera, Volvox carteri f. nagariensis 

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).

Selected references Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv.
Naranjo et al. (2015). The chloroplast NADPH thioredoxin reductase C, NTRC, controls non-photochemical quenching of light energy and photosynthetic electron transport in Arabidopsis. Plant Cell Environ. 2015 Oct 17. doi: 10.1111/pce.12652.
Dwyer et al. (2012). Antisense reductions in the PsbO protein of photosystem II leads to decreased quantum yield but similar maximal photosynthetic rates. J. Ex. Bot. 63(13):4781-95.

Application example


10 ug of chlorophyll/well of Chlamydomonas reinhardtii total cell extract (1), Chlamydomonas reinhardtii subunit gamma deletion mutant thylakoid membrane fraction (2), Arabidospsis thaliana thylakoid membrane fraction (3), Chlamydomonas reinhardtii thylakoid membrane preparation (4) were separated on 12-18% acrylamide-8M urea gel and blotted to nitrocellulose membrane. Filters were blocked 1 h with 5% dry milk in 1 x PBS and probed with anti-ATP synthase subunit gamma antibody (AS08 312, 1: 25 000, 1h) and secondary HRP-conjugated anti-rabbit antibody (1: 10 000, 1 h) in 1 x PBS containing 5% dry milk. All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare), exposure time 30’’ and 3 min (overexposed).

Arabidopsis membrane preparation has been done according to Lezhneva et al. (2008) A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts. J Biol. Chem., 283:24608-24616 and Chlamydomonas membranes were prepared according to Chua & Bennoun (1975) Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. PNAS 72:2175-2179

Courtesy Dr. Yves Choquet

 

Western blot image

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