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Cyt c6 | Thylakoid lumen cytchrome c6 protein (algal)
AS06 202 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
compartment marker of thylakoid lumen
- Product Info
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Immunogen: GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 15 kDa
- Reactivity
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Confirmed reactivity: Chlamydomonas reinhardtii Predicted reactivity: Chlorella vulgaris, Cladophora glomerata, Pediastrum duplex
Species of your interest not listed? Contact usNot reactive in: Arabidopsis thaliana, cyanobacteria, Porphyridium purpureum - Additional Information
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Additional information: Cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii
This product can be sold containing proclin if requested.Additional information (application): Note: antibody will work better with nitrocellulose than with PVDF membrane, Recommended load per well min, 6 µg, Primary antibody incubation needs to be done over night, For detection image please refer to the reference below - Background
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Background: Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized. - Product Citations
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Selected references: Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS06 122 | Clonality: Polyclonal | Host: Rabbit | Reactivity: C.reinhardtii, purple bacteria (CRD1) and plants (CHL27) including A.thaliana, H.vulgare, N. tabacum, P.sativum, P. patens, Synechocystis sp. PCC6803
compartment marker of chloroplast thylakoid and envelope membranes
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)