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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)


- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

AS06 119 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. reinhardtii, C. quitensis Kunt Bartl, C. pumilum,  H. vulgare, N. gaditana, N. tabacum, P. patens, S. martensi, Synechocystis 6803 substrain PCC-M

Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal) in the group Plant/Algal Antibodies / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS06 119)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Info: Read reviews
product information
Background

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Immunogen

GST fusion to cytochrome f from Chlamydomonas reinhartii P23577

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS08 306 | anti-cytochrome f (with reactivity to cyanobacteria)

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

Plant and algal protein extraction buffer

Secondary antibodies

Additional information Contains 0.02 % sodium azide as preservative.
application information
Recommended dilution 1 : 2000-1 : 50 000 (WB)
Expected | apparent MW

34 | 31-32 kDa

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, CRaterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Physcomitrella patens, Selaginella martensi, Synechocystis sp. 6803 substrain PCC-M
Predicted reactivity Algae, Citrus x limon, Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.
Selected references Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.
Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Zou et al. (2017). An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle. Plant Physiol. 2017 Jul;174(3):1334-1347. doi: 10.1104/pp.17.00493.
Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. (Synechocystis PCC6803) Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Ferroni et al. (2016). Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both photosystems. New Phytol. 2016 Apr 5. doi: 10.1111/nph.13939.
Suorsa et al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004.
Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
Dang et al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.

application example

 

Chlamydomonas reinhardtii samples are lysed cells, Arabidopsis thaliana samples are prepared from thylakoid membranes; Samples were boiled 1 min in 3%SDS,then 10ug of chlorophylls were loaded in each well of a 12% acrylamide gel.

 

Western blot detection using anti-cytochrome f antibodies

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