Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)

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AS08 306 | Clonality: Polyclonal | Host: Rabbit | reactiviy: A. thaliana, H. vulgare, T. elongatus, P.abies, S. lycopersicumn, Zea mays


24 st
Item No:
AS08 306

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product information

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.


maize cytochrome f purified from chloroplasts, including a final gel purification on a denaturing gel. protein used to elicit this antibody is conserved in Arabidopsis thaliana cyt f P56771, AtCg00540

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunogold (IG), Western blot (WB)
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AS06 119 | anti-cytochrome f (PetA) rabbit antibody

AS07 231 | anti-cytochrome f (PetA) rabbit antibody

Additional information
application information
Recommended dilution 1 : 120 (IG), 1 : 2500-1 : 5000 (WB)
Expected | apparent MW

31-32 kDa

Confirmed reactivity Arabidopsis thaliana, Echinochloa crus-galli, Hordeum vulgare, Nicotiana tabacum, Panicum miliaceum, Picea abies, Thermosynechococcus elongatus, Solanum lycopersicum, Zea mays
Predicted reactivity Brachypodium distachyon, Cannabis sativa, Cucumis melo, Oryza sativa, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Renato et al. (2014). Tomato fruit chromoplasts behave as respiratory bioenergetic organelles during ripening. Plant Physiol. 2014 Aug 14. pii: pp.114.243931. (western blot, immunogold)

Application example

Western blot using anti-PetA antibodies

1.0 µg of chlorophyll from pea (C3 plant) and from mesophyll (M) and bundle sheath (BS) thylakoids of various treatments of Zea mays, Echinochloa crus-galli, Panicum miliaceum (C4 plants) extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA. Samples were denatured with Laemmli buffer at 75 0C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 40C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602, Lot 1711) diluted to 1:25 000 in 1 % milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 125 seconds.

Courtesy of Dr. Wioleta Wasilewska-Dębowska, Warsaw University, Poland

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