Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (higher plants)
AS08 306 | Clonality: Polyclonal | Host: Rabbit | reactiviy: A. thaliana, H. vulgare, T. elongatus, P.abies, S. lycopersicumn, Zea mays
|Recommended dilution||1 : 120 (IG), 1 : 2500-1 : 5000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Echinochloa crus-galli, Hordeum vulgare, Nicotiana tabacum, Panicum miliaceum, Picea abies, Thermosynechococcus elongatus, Solanum lycopersicum, Zea mays|
|Predicted reactivity||Brachypodium distachyon, Cannabis sativa, Cucumis melo, Oryza sativa, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Renato et al. (2014). Tomato fruit chromoplasts behave as respiratory bioenergetic organelles during ripening. Plant Physiol. 2014 Aug 14. pii: pp.114.243931. (western blot, immunogold)
1.0 µg of chlorophyll from pea (C3 plant) and from mesophyll (M) and bundle sheath (BS) thylakoids of various treatments of Zea mays, Echinochloa crus-galli, Panicum miliaceum (C4 plants) extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA. Samples were denatured with Laemmli buffer at 75 0C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 overnight at 40C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602, Lot 1711) diluted to 1:25 000 in 1 % milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 125 seconds.
Courtesy of Dr. Wioleta Wasilewska-Dębowska, Warsaw University, Poland
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