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AGO set
Tag Antibody Set 

FDX1 | Ferredoxin

AS06 121  | Clonality: Polyclonal  | Host: Rabbit  | Reactivity: A. thaliana, H. vulgare,  P. strobus, S. oleracea, Ch. reinhardtii, Synechocystis 6803 substrain PCC-M

FDX1 | Ferredoxin  in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS06 121)

PRODUCT INFORMATION IN PDF

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272 €
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Datasheet Product citations Protocols Add review

Product Information

Immunogen

Native ferredoxin purified from Spinacia oleracea.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

10 (Spinacia oleracea); 16.7 (Arabidopsis thaliana), 13.7 (Chlamydomonas reinhardtii)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Pinus strobus, Spinacia oleracea, Synechocystis 6803 substrain PCC-M
Predicted reactivity

Dicots, Nicotiana tabacum, Physcomitrella patens

Not reactive in

Phaeodactylum tricornutum 

Application examples

Application examples

application example

western blot detection using anti-ferredoxin antibodies

20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 %  acrylamide gel with 6 M urea. Filters were blotted on PVDF, blocked (1 h) with 5 % milk, incubated with 1: 1000 anti-ferredoxin (over night in 4ºC) in 1 % milk/TBS-T) followed by incubation with 1: 10000 secondary antibody (2 h) coupled to HRP and visualization with standard ECL. 

western blot using anti-Fdx antibodies on Chlamydomonas extracts
20 or 40 µg of total protein from Arabidopsis thaliana (WT) leafs or ferredoxin mutant fd2 were separated on 15 %  acrylamide gel with 6 M urea. Filters were blotted on PVDF,5 µg of soluble protein extracts from Chlamydomonas reinhardtii strain CC-4532 were separated on 15 % SDS-PAGE and blotted at 4 W for 30 min to nitrocellulose membrane (0.1 µm pore size) using semi-dry transfer. The blot was blocked with 1 % non-fat dry milk overnight at room temperature (RT) with agitation. The blot was incubated in the primary antibody solution at a dilution of 1: 10 000 in PBS-T+1% milk for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in PBS-T at RT with agitation. The blot was incubated in secondary antibody solution (anti-rabbit IgG-AP conjugated) diluted to 1: 300 in PBS-T+1% milk for 1h at RT with agitation. The blot was washed as above and developed with exposure time of 2.5 minutes.

Courtesy of Dr. Marcus Miethke, Department of Chemistry & Biochemistry, UCLA

Additional information

Additional information

In Arabidopsis thaliana leaf extracts there is a strong cross-reactivity at 20 kDa.

Load per well: 20-40  µg/lane required for Arabidopsis thaliana, 2-10 µg for other species.

This product can be sold containing proclin if requested.

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Background

Background

Ferredoxins are acidic, low molecular weight, soluble iron-sulfur proteins found in various organisms. Iron-sulfur proteins are defined as proteins carrying iron-sulfur cluster(s) in which the iron is at least partially coordinated by sulfur. The protein acts as multifunctional electron carriers in diverse redox systems. The chloroplast ferredoxin is involved in both cyclic and non-cyclic photophosphorylation reactions of photosynthesis and other reductive reactions in the chloroplast.

Product citations

Selected references Cvetkovska et al. (2018). Characterization of photosynthetic ferredoxin from the Antarctic alga Chlamydomonas sp. UWO241 reveals novel features of cold adaptation. New Phytol. 2018 Jul;219(2):588-604. doi: 10.1111/nph.15194.
Jokel et al. (2018). Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. Plant J. 2018 Mar 25. doi: 10.1111/tpj.13897.
Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010.
Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.
Higuchi et al. (2011). Modulation of macronutrient metabolism in barley leaves under iron-deficient condition. Soil Sc and Plant Nutr. 57 (2): 233-247.

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