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Tag Antibody Set 

95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)

AS08 281  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity:Cyanobacteria and red algae

95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore) in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS08 281)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

native protein purified from phycobilisomes of Porphyridium cruentum

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 300-1 : 1000 (WB)
Expected | apparent MW

95 kDa (Porphyridium cruentum), 75-120 kDa (other species)

Reactivity

Confirmed reactivity Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ATCC 27264,  PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
Predicted reactivity Algae (red), Cyanobacteria
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost.
Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.

Related products

Related products

AS08 277 | Anti-APC | allophycocyanin alpha and beta, rabbit antibodies

AS08 278 | Anti-R-PC | R-phycocyanin, rabbit antibodies

AS08 279 | Anti-b-PE | phycoerythrobilin, rabbit antibodies

AS08 280 | Anti-B-PE | phycoerythrobilin and phycourobilin, rabbit antibodies

Background

Background

A large polypeptide Lcm with a few phycocyanobilin chromophores which acts as a terminal energy acceptor and as a linker polypeptide between the phycobilisomes and the photosynthetic reaction centres (PS2).

Product citations

Selected references Redlinger & Gantt (1982) A Mr 95,000 polypeptide in Porphyridium cruentum phycobilisomes and thylakoids: PNAS 79:5542.
Redlinger & Gantt (1981). Phycobilisome structure of Porphyridium cruentum. Plant Physiol. 68:1375.

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