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AGO set
Tag Antibody Set 

APC | allophycocyanin alpha and beta

AS08 277  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity: Red algae, cyanobacteria

APC | allophycocyanin alpha and beta in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS08 277)

PRODUCT INFORMATION IN PDF

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355 €

Datasheet Product citations Add review

Product Information

Immunogen

native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Recommended dilution 1 : 200-1 : 500 (IL), 1 : 1500 - 3000 (WB)
Expected | apparent MW

14-19 kDa

Reactivity

Confirmed reactivity Porphyridium cruentum, Synechocystis PCC 6803
Predicted reactivity Red Algae, Cyanobacteria
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Related products

Related products

AS08 278 | anti-R-PC | R-phycocyanin

AS08 279 | anti-b-PE | phycoerythrobilin

AS08 280 | anti-B-PE | phycoerythrobilin and phycourobilin

Background

Background

Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.

Product citations

Selected references Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.
Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)
Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18