HliD | High light inducible protein

AS10 1610 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803
HliD | High light inducible protein in the group Plant/Algal Antibodies / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS10 1610)


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product information
Background HliD (ScpE) protein, high light inducible protein, is LHC-similar stress-induced protein from cyanobacteria. HliD is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
Immunogen Synthetic peptide (amino acids 15 – 30) for HliD protein from Synechocystis sp. PCC 6803. NP_440269.1
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid
Quantity 100 ĩl
Storage Store short-term 4°C. Long term -20°C. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide.
Tested applications Western blot (WB)
Related products

AS10 1615 | nti-HliD | High light inducible protein, larger pack size

Secondary antibodies

Additional information

Using nitrocellulose for western blotting is highly recommended to obtain a signal with this antibody.

Pre-immune serum is available to this product upon request.

application information
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

5 kDa

Confirmed reactivity Synechocystis sp. PCC 6803
Predicted reactivity According to sequence analysis antibody may react with homologous Hli protein(-s) from Anabaena, Thermosynechococcus, Gloeobacter, Prochlorococcus, Synechococcus and Crocosphaera.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Staleva et al. (2015). Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins. Nat Chem Biol. 2015 Apr;11(4):287-91. doi: 10.1038/nchembio.1755.
Chidgey et al. (2014). A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase. Plant Cell. 2014 Mar;26(3):1267-79. doi: 10.1105/tpc.114.124495.
Knoppová et al. (2014). Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis. Plant Cell. 2014 Mar;26(3):1200-12. doi: 10.1105/tpc.114.123919. Epub 2014 Mar 28.

application example

western blot using HilD (ScpE) antibody in Synechocystis
40 µg of membrane proteins from Synechocystis 6803 WT and DhliD strains  was separated on 12-20 % SDS-PAGE and blotted 3h to PVDF.  Blot was blocked with  0.2% Tween 20 in TBS for 1h at room temperature with agitation and than incubated in the primary antibody at a dilution of 1: 2 000 overnight at 10°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 5 min in TBS-T at room temperature with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in  for 1h at room temperature with agitation. Finally, the blot was washed as above, developed for 5 min with ECL (Plus Supersignal West Pico) according to the manufacturers instructions and exposed for 30 min in LAS 4000 imager.

WT and ΔScpE deletion mutants grown at 40 μE m-2s-1 and exposed to HL 2000 μE m-2s-1 for 30 min. 

Courtesy of Dr. Josef Komenda, Academy of Science of Czech Republic

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