Lhc1 | PS 1 light harvesting holo-complex
AS08 282 | Clonality:Polyclonal | Host:Rabbit | Reactivity:Diatoms
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Purified LHC complex from Porphyridium cruentum
2 µg of total chlorophyll/lane of total cell extract from (1) Thalassiosira pseudonana, (2) Heterosigma akashiwo , (3) Guillardia theta, (4) Aureococcus anophagefferens, extracted with Agrisera protein extraction buffer PEB, were separated on 13-17% SDS-PAGE and blotted 2h to nitrocellulose. membranes were blocked 1h with 5% low-fat milk powder in PBS-T (0.1% TWEEN 20) and
The light-harvesting system of higher plant is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.
Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566