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Agrisera Super Deal

Primary/Secondary/ECL



Add only 20 € to your primary antibody purchase
and you will also receive:

- Secondary antibody
Goat anti-rabbit, HRP conjugated
(to be used >1: 25 000)

- Two chemiluminescent detection reagents
Agrisera ECL Bright/SuperBright,  for 10 midi blots (picogram and femtogram detection range)

AS18 PrimarySecondaryECL

Lhca3 | PSI type III chlorophyll a/b-binding protein

AS01 007  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Monocots and Dicots including: A.thaliana, H.vulgare, O. sativa, P. patens, P. sativum, P. vulgaris, S.oleracea

Lhca3 | PSI type III chlorophyll a/b-binding protein in the group Plant/Algal Antibodies / Photosynthesis / LHC at Agrisera AB (Antibodies for research) (AS01 007)

PRODUCT INFORMATION IN PDF

Qty: 
332 €

Info: Read reviews
product information
Background

The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.
A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
Immunogen

BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies

AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas

Available antibodies against pigment-binding proteins- LHC

recommended secondary antibody

Plant protein extraction buffer

Secondary antibodies
Additional information
application information
Recommended dilution 1 : 2000-1 : 5000 (WB)
Expected | apparent MW

29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)
Confirmed reactivity Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Triticum aestivum, Zea mays
Predicted reactivity Dicots, Gymnosperms
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information Protein is processed into mature form (Jansson 1999).
Selected references Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.
Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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