Lhca3 | PSI type III chlorophyll a/b-binding protein

AS01 007 | Clonality: Polyclonal | Host: Rabbit | Reactivity: monocots and dicots including: A.thaliana, H.vulgare, O. sativa, P. patens, P. sativum, P. vulgaris, S.oleracea

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Background		The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK. A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
Immunogen		BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Total IgG
Format		Lyophilized in PBS pH 7.4
Quantity		200 µl
Reconstitution		For reconstitution add 200 µl of sterile water
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS01 011 \| A set of 10 plant anti-Lhca and anti-Lhcb antibodies AS01 011 Chlamydomonas \| A set of anti-Lhc antibodies for Chlamydmonas Available antibodies against pigment-binding proteins- LHC recommended secondary antibody Plant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 2000-1 : 5000 (WB)
Expected \| apparent MW		29 \| 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)
Confirmed reactivity		Arabidopsis thaliana, Aracis hypogae, Bryopsis corticulans, Hordeum vulgare, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Spinacia oleracea, Triticum aestivum, Zea mays
Predicted reactivity		Dicots, Gymnosperms
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Protein is processed into mature form (Jansson 1999).
Selected references		Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul. Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot) Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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