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AGO set
Tag Antibody Set 

Lhcb1 | LHCII type I chlorophyll a/b-binding protein

AS01 004 | Clonality: Polyclonal | Host: Rabbit  | Reactivity: Photosynthetic eukaryotes including A. thaliana, A. hypogaea, Ch. vulgaris, C. quitensis Kunt Bartl, C. pumilum, H. vulgare, L. esculentum (Solanum lycopersicon), M. crystallinum, N. tabacum, O. sativa, P. sativum, P. vulgaris, R. discolor, S. alaba, S. vulgaris, S. oleracea, T. aestivum, Z. mays 

Lhcb1 | LHCII type I chlorophyll a/b-binding protein in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS01 004)

PRODUCT INFORMATION IN PDF

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Product Information

Immunogen

BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430  (Lhcb1.4), and At2g34420 (Lhcb1.5)

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

28 | 25 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Triticum aestivum, Zea mays
Predicted reactivity Aegilops tauschii, Catalpa bungei, Cucumis sativus, Brachypodium distachyon, Lotus japonicus, Hordeum vulgare, Musa acuminata, Nicotiana tabacum, Physcomitrella patens, Solanum tuberosum, Zpsteria marina, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-Lhcb1 antibody

10 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Zea mays leaf, (4) Chlamydomonas reinhardtii total cell, (5) Spinacia oleracea total leaf, (6) Physcomitrella patens, (7) Solanum tuberosum total leaf, (8) Solanum esculentum total leaf, all extracted with Protein Extraction Buffer PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2-2.5 % blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, recommended secondary antibody AS09 602) diluted to 1:25 000 in TBS-T for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescent detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 2 minutes.

Additional information

This antibody is provided as a total IgG fraction, e.g. serum purified on Protein G to total immunoglobulin.

Lhcb1 Protein is processed into mature form (Jansson 1999).

Related products

Related products

AS01 004 | Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, rabbit antibodies

AS01 004PRE | Lhcb1 | LHCII type I chlorophyll a/b-binding protein, pre-immune serum for control in immunolocalization

AS01 011 | 2 | Set of 10 plant anti-Lhca and anti-Lhcb rabbit antibodies

AS01 011 CHLAMYDOMONAS | 2 | Set of 4 Chlamydomonas anti-Lhc rabbit antibodies

Plant protein extraction buffer

Background

Background

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII:  Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476

Product citations

Selected references Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.
Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Rantala et al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.
Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.
Yang-Er Chen
et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.

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