Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated
AS13 2704 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
|Info:||More information||Read reviews|
|Recommended dilution||1 : 10 000 (WB)|
|Expected | apparent MW||
25 | 25 kDa for Arabidopsis thaliana
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Solanum lycopersicum, Spinacia oleracea, Zea mays|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.
Rantala et al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.
Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218. Schönberg et al. (2017). Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression. Plant J. 2017 Jun;90(6):1176-1186. doi: 10.1111/tpj.13536. Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.
Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.
Jia et al. (2014). Accumulation of NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana. Plant J. 2014 Dec 30. doi: 10.1111/tpj.12753.
Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.
1 ug of thylakoid membranes isolated from Arabidopsis thaliana wilde-type and mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 1 µg of total chlorophyll was loaded and separated on 16% SDS-PAGE, and then blotted for 2 h onto nitrocellulose membrane. Blots were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS01 004, 1: 5 000) for 2.5 h, which was then decanted and the blot was washed 3 times for 5 min in TBST. Membrane was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h, followed by washing steps as above. All the steps fallowing transfer were performed in room temperature (RT) with agitation. Membrane was developed for 5 min with ECL according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 30 s increment time for around 5 min.
Courtesy of a phd candidate Małgorzata Pietrzykowska, Umeå Plant Science Centre, Sweden
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